hairy cell leukemia

A Case of Hairy Cell Leukemia Diagnosed Simultaneously with Lymphoplasmacytic Lymphoma by Anti-CD Antibody Microarray Method

LYMPHOID TUMORS , , , , ,

AN Khvastunova1,2, LS Al-Radi3, OS Fedyanina1,2, SA Lugovskaya4, SA Kuznetsova1,2

1 Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 1 Samory Mashela str., Moscow, Russian Federation, 117997

2 Center for Theoretical Problems of Physicochemical Pharmacology, 4 Kosygina str., Moscow, Russian Federation, 119991

3 National Medical Hematology Research Center, 4а Novyi Zykovskii pr-d, Moscow, Russian Federation, 125167

4 Russian Medical Academy of Postgraduate Education, 2/1 Barrikadnaya str., Moscow, Russian Federation, 1125993

For correspondence: Alina Nikolaevna Khvastunova, PhD in Biology, 1 Samory Mashela str., Moscow, Russian Federation, 117997; Tel.: +7(495)287-65-70; e-mail: alina_shunina@mail.ru

For citation: Khvastunova AN, Al-Radi LS, Fedyanina OS, et al. A Case of Hairy Cell Leukemia Diagnosed Simultaneously with Lymphoplasmacytic Lymphoma by Anti-CD Antibody Microarray Method. Clinical oncohematology. 2019;12(3):243–6 (In Russ).

doi: 10.21320/2500-2139-2019-12-3-243-246

ABSTRACT

The paper deals with a combined case of hairy cell leukemia and lymphoplasmacytic lymphoma with IgMκ paraprotein secretion. The use of anti-CD antibody microarray enabled the simultaneous assessment of immunophenotype as well as morphological and cytochemical analysis. Small populations of hairy (3 % of total lymphocyte count) and plasma (2 %) cells including Mott cells (0.2 %) were found in the peripheral blood of a patient with leukopenia. The results obtained by the anti-CD antibody microarray method speeded up provisional diagnosis which was later confirmed by standard diagnostic methods.

Keywords: anti-CD antibody microarray, hairy cell leukemia, lymphoplasmacytic lymphoma, hairy cells, plasma cells, Mott cells.

Received: November 12, 2018

Accepted: May 2, 2019

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REFERENCES

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    [Khvastunova AN, Al-Radi LS, Kapranov NM, et al. Cell-binding microarray application in diagnosis of hairy cell leukemia. Oncohematology. 2015;10(1):37–45. doi: 10.17650/1818-8346-2015-1-37-45. (In Russ)]

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Role of Superficial CD200 Marker in Differential Diagnosis of Malignant B-Cell Lymphoproliferative Diseases

LYMPHOID TUMORS , , , , ,

YuV Mirolyubova, EA Stadnik, TS Nikulina, VV Strugov, TO Andreeva, YuV Virts, RV Grozov, AYu Zaritskey

Federal Almazov North-West Medical Research Centre, 2 Akkuratova str., Saint Petersburg, Russian Federation, 197341

For correspondence: Yuliya Vladimirovna Mirolyubova, 2 Akkuratova str., Saint Petersburg, Russian Federation, 197341; e-mail: juli9702@yandex.ru

For citation: Mirolyubova YuV, Stadnik EA, Nikulina TS, et al. Role of Superficial CD200 Marker in Differential Diagnosis of Malignant B-Cell Lymphoproliferative Diseases. Clinical oncohematology. 2017;10(2):169–75 (In Russ).

DOI: 10.21320/2500-2139-2017-10-2-169-175

ABSTRACT

Background & Aims. Flow cytometry is successfully used for diagnosis of malignant lymphoproliferative disorders. However, there are atypical cases that are difficult to interpret; thus, new markers relevant for the differential diagnosis are to be searched for. The aim is to analyze CD200 expression in patients with B-cell lymphoproliferative disorders.

Materials & Methods. 187 patients with chronic lymphocytic leukemia (CLL), 14 patients with mantle cell lymphoma (MCL), 9 patients with marginal zone lymphoma (MZL), and 5 patients with hairy cell leukemia (HCL) were enrolled in the study. Neoplasm was not confirmed in 12 subjects. The patients underwent the following tests: CBC, immunophenotyping of peripheral blood or bone marrow lymphocytes, and a cytogenetic test. In some cases, an additional immunohistochemical test of bone marrow trepanobiopsy or lymph node biopsy samples was required.

Results. In all cases of CLL and HCL, the CD200 expression was positive; mean fluorescence intensity was higher in these cases as compared to other groups. Negative expression of CD200 prevailed in MCL patients; however, at the same time 2 cases of intermediate and positive expression were reported, both showing moderate fluorescence intensity values. CD200 expression was heterogeneous in MZL patients.

Conclusion. The CD200 negative expression excludes typical HCL and CLL. Additional cytogenetic and immunnohistoсhemical tests should be performed in such cases to verify the diagnosis, first of all, MCL or MZL.

Keywords: CD200, flow cytometry, diagnosis, chronic lymphocytic leukemia, mantle cell lymphoma, marginal zone lymphoma, hairy cell leukemia.

Received: September 7, 2016

Accepted: January 3, 2017

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