pro- and antifibrotic factors

Pro- and Antifibrotic Factors in the Serum of Patients with Chronic Myeloproliferative Disorders

MYELOID TUMORS , , , ,

AA Silyutina, NM Matyukhina, EG Lisina, VI Khvan, SN Leleko, NT Siordiya, OV Sirotkina, PA Butylin

VA Almazov National Medical Research Center, 2 Akkuratova str., Saint Petersburg, Russian Federation, 197341

For correspondence: Pavel Andreevich Butylin, PhD, 2 Akkuratova str., Saint Petersburg, Russian Federation, 197341; e-mail: butylinp@gmail.com

For citation: Silyutina AA, Matyukhina NM, Lisina EG, et al. Pro- and Antifibrotic Factors in the Serum of Patients with Chronic Myeloproliferative Disorders. Clinical oncohematology. 2017;10(4):479–84 (In Russ).

DOI: 10.21320/2500-2139-2017-10-4-479-484

ABSTRACT

Background. The study of pro- and antifibrotic factors in the serum of patients with Ph-negative chronic myeloproliferative disorders (CMPDs) will allow to understand better the mechanisms of myelofibrosis development, as well as to identify new diagnostic markers.

Aim. To assess the correlation between the levels of classic (TGF-β, bFGF, MMP-2, -9, -13 and VEGF) and new proinflammatory serum factors (galectin-3), involved into development of myelofibrosis in different Ph-negative forms of CMPDs and genetic abnormalities.

Materials & Methods. The research included 55 CMPD patients (13 with polycythemia vera, 17 with essential thrombocythemia, 25 with primary myelofibrosis) and 8 healthy controls. Whole blood genomic DNA extraction was used to evaluate mutations JAK2V617F, CALR (deletions and insertions), MPLW515L, and MPLW515K. Antibody-immobilized ELISA was used to evaluate the levels of galectin-3, TGF-β, bFGF, VEGF, MMP-2, MMP-9 and MMP-13.

Results. The analysis showed the differences in serum MMP-9, VEGF, TGF-β and galectin-3 levels in patients with different CMPDs. A tendency towards the decrease of serum MMP-9 levels in patients with CALR mutations was shown.

Conclusion. The shown differences between patients with different CMPDs may serve as a basis for improving diagnostic protocols in challenging differential diagnosis of CMPDs.

Keywords: Ph-negative chronic myeloproliferative disorders, pro- and antifibrotic factors, JAK2V617F, CALR, MPLW515L, MPLW515K, MMP-2, MMP-9, MMP-13, galectin-3.

Received: April 26, 2017

Accepted: July 5, 2017

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