clonality

PCR-based clonality detection in angioimmunoblastic T-cell lymphoma

CLINICAL PRESENTATION, DIAGNOSIS, AND MANAGEMENT OF LYMPHOID MALIGNANCIES , , ,

Yu.V. Sidorova, Ye.Ye. Nikulina, N.G. Chernova, L.G. Gorenkova, Ye.A. Gilyazitdinova, S.K. Kravchenko, A.M. Kovrigina, and A.B. Sudarikov

Hematology Research Center, RF Ministry of Health, Moscow, Russian Federation

ABSTRACT

In this article, we discuss the issues of angioimmunoblastic T-cell lymphoma diagnosis, particularly the PCR-based methods of clonality detection. Assessments of T-and B-cell clonality was based on TCRG (Vg-Jg), TCRB (Vb-Jb, Db-Jb), IGH (FR1, FR2, and FR3), IGK (Vk-Jk, Vk/intron-Kde), or IGL (Vl-Jl) gene rearrangements in 15 patients. Clonal TCRG gene rearrangements were found in 66.7 % of primary biopsy samples. The combined use of primers for TCRG and TCRB gene rearrangements confirmed T-cell monoclonal population in most cases (86.7 %). The rate of B-cell clonality detection was 26.6 %. The presence of B-cell clones was not associated with monoclonal secretion in the blood or detecting Epstein-Barr virus positive B-cells in the biopsy samples. PCR-based clonality analysis is an important step in diagnosis of angioimmunoblastic T-cell lymphoma that enables identifying monoclonal origin of T-lymphocytes in most cases. However, when interpreting the results obtained by this method, it is necessary to consider the possibility of detecting B-cell monoclonal products of unclear origin.

Keywords: angioimmunoblastic T-cell lymphoma, PCR, clonality, T-cell antigen receptor

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