Immunophenotypical and cytogenetic features of tumor cells in a patient with chronic lymphocytic leukemia associated with prolonged exposure to irradiation

S.N. Kolyubaeva1, I.A. Sukhina1, V.Yu. Nikitin1, T.V. Isakova1, A.S. Polyakov1, S.N. Malakhova1, N.V. Il’yin2, J.N. Vinogradova2, and L.А. Myakoshina2

1 Military Medical Aсademy, Saint Petersburg, Russian Federation

2 Russian Research Centre for Radiology and Surgical Technologies, RF Ministry of Health, Saint Petersburg, Russian Federation


ABSTRACT

In this article, we describe immunoрhenotypical and cytogenetic features of tumor cells in the patient with chronic lymphocytic leukemia (CLL) and prolonged exposure to irradiation in the history. Finding of 0.67% to 0.73% of metaphases with dicentric chromosomes confirmed the effect of radiation in this patient. Chromosome banding with mitogen stimulation of peripheral lymphocytes revealed 2% of cells with 47,XY, +12 karyotype, while FISH detected the greater number of cells with trisomy 12 in interphase bone marrow cells and peripheral lymphocytes. Immunophenotyping of bone marrow cells revealed the difference from “classic” CCL, i.e. simultaneous low-positive CD22 and CD79b expression as well as CD38 expression on the surface of tumor cells.


Keywords: chronic lymphocytic leukemia, fluorescence in situ hybridization, trisomy 12, irradiation, radiation-specific damage.

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